Coding

Part:BBa_K1964000:Design

Designed by: Patrick Gerlinger   Group: iGEM16_Marburg   (2016-10-14)


[PanD] Aspartate 1-decarboxylase from C. glutamicum [Codon optimized]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Seqeuencing of panD in the operon plasmid panD sequencing alignment
Sequencing of panD as part of the operon plasmid that was designed as part of our project. We used primers that bind in the adjacent gene and in the backbone pACYC184.
Operon plasmid map

Operon plasmid map

Plasmid map of the operon plasmid that was designed as part of our project.

Design Notes

While optimized for codon usage in E. coli we had to consider the biobrick restriction sites of EcoRI, PstI, XbaI, SpeI. We manually altered the sequence to avoid these cut sites.


Source

Synthesized DNA Sequence.

References